ebc buffer Search Results


ebc buffer  (Beijing CWBio)
90
Beijing CWBio ebc buffer
Ebc Buffer, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebc+buffer/pm39900908-89-6-14?v=Beijing+CWBio
Average 90 stars, based on 1 article reviews
ebc buffer - by Bioz Stars, 2026-06
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ebc lysis buffer  (Applichem inc)
90
Applichem inc ebc lysis buffer
Ebc Lysis Buffer, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebc+buffer/pm26563945-68-15-23?v=Applichem+inc
Average 90 stars, based on 1 article reviews
ebc lysis buffer - by Bioz Stars, 2026-06
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ebc buffer  (Topscience Co Ltd)
90
Topscience Co Ltd ebc buffer
Ebc Buffer, supplied by Topscience Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebc+buffer/pm37341611-41-6-24?v=Topscience+Co+Ltd
Average 90 stars, based on 1 article reviews
ebc buffer - by Bioz Stars, 2026-06
90/100 stars
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ebc buffer  (Kodak)
86
Kodak ebc buffer
FIG. 3. Mapping the cyclin D binding domains in DMP1. (A) Interactions between DMP1 and cyclin D2 in insect cells. Sf9 cells were coinfected with baculoviruses encoding either wild-type or mutant DMP1 together with murine cyclin D2. Cells metabolically labeled with [35S]methionine were disrupted, and cleared lysates were precipitated with <t>anti-Flag</t> <t>M2</t> beads or with a control antibody (designated C), as indicated at the bottom of the panel. The position of coprecipitating cyclin D2 is indicated in the right margin. DMP1 mutants were normalized for methionine content to ensure roughly equivalent protein inputs. The one exception was mutant M13, which contains only a single methionine residue and was overloaded; despite the high input concentration of M13, it did not interact strongly with cyclin D2, reinforcing the central conclusions (see text). (B) Binding assays performed in transfected NIH 3T3 cells. NIH 3T3 cells cotransfected with pFLEX-DMP1 and pBJ5-cyclin D2 expression vectors were lysed in <t>EBC</t> buffer, and cleared lysates immunoprecipitated with M2 beads as indicated below the panel were immunoblotted with monoclonal antibodies to cyclin D2 (top). Lysates from the same transfections (one-sixth of the amounts used for immunoprecipitations) were directly blotted with anti-D2 to confirm equal expression of the cyclin in all transfections (bottom). The position of cyclin D2 is indicated to the right.
Ebc Buffer, supplied by Kodak, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebc+buffer/10__1128_slash_mcb__18__3__1590-96-23-50?v=Kodak
Average 86 stars, based on 1 article reviews
ebc buffer - by Bioz Stars, 2026-06
86/100 stars
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Image Search Results


FIG. 3. Mapping the cyclin D binding domains in DMP1. (A) Interactions between DMP1 and cyclin D2 in insect cells. Sf9 cells were coinfected with baculoviruses encoding either wild-type or mutant DMP1 together with murine cyclin D2. Cells metabolically labeled with [35S]methionine were disrupted, and cleared lysates were precipitated with anti-Flag M2 beads or with a control antibody (designated C), as indicated at the bottom of the panel. The position of coprecipitating cyclin D2 is indicated in the right margin. DMP1 mutants were normalized for methionine content to ensure roughly equivalent protein inputs. The one exception was mutant M13, which contains only a single methionine residue and was overloaded; despite the high input concentration of M13, it did not interact strongly with cyclin D2, reinforcing the central conclusions (see text). (B) Binding assays performed in transfected NIH 3T3 cells. NIH 3T3 cells cotransfected with pFLEX-DMP1 and pBJ5-cyclin D2 expression vectors were lysed in EBC buffer, and cleared lysates immunoprecipitated with M2 beads as indicated below the panel were immunoblotted with monoclonal antibodies to cyclin D2 (top). Lysates from the same transfections (one-sixth of the amounts used for immunoprecipitations) were directly blotted with anti-D2 to confirm equal expression of the cyclin in all transfections (bottom). The position of cyclin D2 is indicated to the right.

Journal: Molecular and Cellular Biology

Article Title: Gene Expression and Cell Cycle Arrest Mediated by Transcription Factor DMP1 Is Antagonized by D-Type Cyclins through a Cyclin-Dependent-Kinase-Independent Mechanism

doi: 10.1128/mcb.18.3.1590

Figure Lengend Snippet: FIG. 3. Mapping the cyclin D binding domains in DMP1. (A) Interactions between DMP1 and cyclin D2 in insect cells. Sf9 cells were coinfected with baculoviruses encoding either wild-type or mutant DMP1 together with murine cyclin D2. Cells metabolically labeled with [35S]methionine were disrupted, and cleared lysates were precipitated with anti-Flag M2 beads or with a control antibody (designated C), as indicated at the bottom of the panel. The position of coprecipitating cyclin D2 is indicated in the right margin. DMP1 mutants were normalized for methionine content to ensure roughly equivalent protein inputs. The one exception was mutant M13, which contains only a single methionine residue and was overloaded; despite the high input concentration of M13, it did not interact strongly with cyclin D2, reinforcing the central conclusions (see text). (B) Binding assays performed in transfected NIH 3T3 cells. NIH 3T3 cells cotransfected with pFLEX-DMP1 and pBJ5-cyclin D2 expression vectors were lysed in EBC buffer, and cleared lysates immunoprecipitated with M2 beads as indicated below the panel were immunoblotted with monoclonal antibodies to cyclin D2 (top). Lysates from the same transfections (one-sixth of the amounts used for immunoprecipitations) were directly blotted with anti-D2 to confirm equal expression of the cyclin in all transfections (bottom). The position of cyclin D2 is indicated to the right.

Article Snippet: For detection of Flag-tagged DMP1 or its complexes with D cyclins, 50 to 100 ml of lysate was diluted with 300 ml of EBC buffer; M2 beads (24 ml of a 1:1 suspension in phosphate-buffered saline on June 21, 2016 by guest http://m cb.asm .org/ D ow nloaded from [PBS] [Kodak]) were added and were recovered by centrifugation after incubation for 2 h at 4°C.

Techniques: Binding Assay, Mutagenesis, Metabolic Labelling, Labeling, Control, Residue, Concentration Assay, Transfection, Expressing, Immunoprecipitation, Bioprocessing